Aim: This study was performed to unmask the haematological and biochemical alterations occurred in blood and serum of infected buffaloes, respectively. Material & Method: A total of 100 blood and serum samples were collected from infected as well as non-infected buffaloes during the ante-mortem examination. Animals were bled from the jugular vein into 10ml vacutainer tubes containing EDTA (Ethylene Diamine Tetraacetic Acid) for the estimation of various haematological parameters and 10 ml blood collected in serum clot activator tube to study biochemical parameters. Result: The infected buffaloes showed a significant reduction in the mean Hb, PCV, and lymphocyte and increased TLC, neutrophil and eosinophil. The monocytes level decreased and basophils levels increased non-significantly in infected buffaloes. In the biochemical profile significantly reduction in the values of total serum protein, total albumin and A:G ratioand increased in AST/ SGOT, total globulin, ALT/ SGPT, acid phosphatase (ACPase), alkaline phosphatase (AKPase) and total bilirubin level reported. Conclusion: Based on the results we got during study it can be conclude that Fasciola spp. infection cause heavy liver and muscle damage and cause heavy loss of blood from the body.