Over 70% of wheat (Triticum aestivum) cultivars grown worldwide have a semidwarf phenotype controlled by the major genes Rht-B1, Rht-D1, and Rht8c. Rht-B1 and Rht-D1 encode for DELLA proteins which act to repress GA-responsive growth by repressing GA-signaling. The mutant alleles Rht-B1b and Rht-D1b confer dwarfism by producing more active forms of DELLA growth repressors. The objective of this study was to determine the frequency of occurrence of Rht-B1b and Rht-D1b genes in some of wheat cultivars grown in India. Sixteen Indian wheat cultivars were evaluated with PCR-based molecular markers for Rht-B1b and Rht-D1b. These PCR-based markers can detect the point mutations responsible for the two major semidwarfing genes Rht-B1b (Rht1) and Rht-D1b (Rht2) in wheat. Fourteen cultivars were found to have both Rht-B1b and Rht-D1b genes whereas two cultivars were identified that did not carry any of the two semi-dwarfing genes.