Jatropha curcas L. is a shrub belonging to Euphorbiaceae family. There is a worldwide interest to cultivate this plant in large scale for the production of biodiesel from its seed oil. Hence, improvement to get commercially profitable quantities of seed yield with high oil content for biodiesel production is the prime objective. Intra-specific hybridization by xenogamy (cross-pollination) was carried out to meet this objective. A total of 50 accessions were identified from the Jatropha germplasm centre of Labland Biotech Private Limited, Mysore for the purpose of hybridization studies. Out of many highly successful crosses made in this study, a maximum of 80% effective fruit formation and 61% quality seed formation were achieved. The efficiency of hybrid seed production is discussed.