This study compared the effect of Problem-Based Learning (PBL) and lecture method on student’s attitude towards biology studying in class ninth. The study was conducted on a sample of 188 students randomly distributed into experimental (n=93, taught through (PBL) problem based learning) and control (n=95, taught through lecture method) group. Both the groups were taught the same topics of biology by the same instructor for a period of 5 weeks. The data was collected via the pre- test and post-test administration of Biology attitude test. The results were statistically analyzed using SPSS software by employing both descriptive and inferential statistics (t-test). Results indicated that There was no significant difference in attitude of students towards biology taught by Problem based learning and lecture method.