Aflatoxins are natural toxins, produced by several fungal species and are secondary metabolites of mould fungi identified in toxigenic molds. The aflatoxin was assessed using the chromatography technique. The aim of the study is to estimate the formulated GSMA and to assess the aflatoxin content. The formulated Nutraceutical supplement was compared with market sample and FSSAI limit. The obtained results satisfied the limit and ensure the safety of the product.