Sartorius is the most superficial extensor muscle of thigh and the longest muscle in the body. It crosses hip as well as knee joint and accordingly act as a flexor on both the joints. During routine dissection class of undergraduate students, unilateral variation in the origin of Sartorius muscle was observed. There were two heads of origin, the major part was originating normally from anterior superior iliac spine (ASIS) and its adjacent area whereas, the accessory tendinous origin took place from inguinal ligament and get attached to main muscle belly, about 5.4 cm below the inguinal ligament. Sartorius muscle flaps are very important to cover complicated wounds in reconstruction surgeries as well as during wound sterilization treatment.