The aim of the present study was to compare two suture patterns used for intestinal anastomosis in terms of their effect on white blood cells (WBCS) count, surgical site infection and mortality rate. Twelve apparently healthy donkeys were used. Animals were divided into two equal groups each of six animals. Two rows serosubmucosal continuous Lambert suture (SSCLS) patterns were performed in the first group while the second group underwent one row serosubmucosal interrupted suture (SSIS) pattern. White blood cell count was measured zero time, 24hr, 48hr and 72hr for determination of changes in the total white blood cell count. Also surgical site infection was observed at surgical site to detect abscesses or inflammatory exudates and mortality rate in each group over four weeks period after surgery. A significant rise (P value ≤ 0.05) was occurred in total white cells count at 48 hours post jejunal anastomosis in SSIS group compared with SSCLS group. Observation at surgical site examination revealed that the surgical site infection showed 50% in SSCLS group versus 66.7% in SSIS group while the mortality rates were found to be 33.3% and 16.7% in SSIS and SSCLS groups respectively. on conclusion, the two rows sero-submucosal continuous Lembert suture (SSCLS)group was found to be superior than the one row sero-submucosal interrupted suture (SSIS) pattern for jejunum anastomosis in donkeys, thus, being safe, reliably and can be performed as a technique for intestinal anastomosis in donkey.