Ipomoea clarkei Hook. f. is rare, climbing herb and restricted to India. The conservation of the rare plants in recent years was extensively done by the reintroduction of in-vitro propagated plantlets to their natural habitat. The Ipomoea clarkei Hook.f.is one of the ignored, rare and an endemic terrestrial Species of Western Ghats demands attention to the conservation. In-vitro propagation using tissue culture is a significant method for rapid multiplication upon the natural germination of seeds and vegetative means. Propagation by using Plant Tissue Culture of such plant aims to conserve the plant by rehabilitation and reduce the pressure on the ecosystem. In present investigation it was observed development of multiple shoots on MS basal medium supplemented with 0.5 mg/lit BAP and combination 0.1 mg/lit NAA from nodal segment.